Sevene has developed several detoxifying formulae from a combination of medicinal plants. These formulae have been tested by university research laboratories in models of intoxication by mixed pollutants present in the environment.
A primary experimental study has demonstrated the protective effects of plant mixtures on cells – Dandelion (Taraxacum officinalis), Barberry (Berberis vulgaris), Burdock (Arctium lappa) – on human HEP G2 hepatocyte lines intoxicated by glyphosate, an active molecule of Roundup®, which is the most widely-used herbicide in the world. A solution of plant mixtures was applied to cell cultures either before or after their intoxication. Cell viability was measured after 24h, 48h and 72h by a test of mitochondrial respiration.
UCell recovery of 89% has been reported for HEP G2 cells when they were exposed to the herbal drug mixture during the 48 hours preceding the exposure to the toxic element, whereas cell viability was 32% when HEP G2 cells were exposed to the toxic element only.
The plant mixture protects hepatocytes against cell death provoked by the herbicide. This study was the subject of a publication in an international magazine : Dig1 protects against cell death provoked by glyphosate-based herbicides in human liver cell lines, Gasnier et al. Journal of Occupational Medicine and Toxicology. 2010, 5:29
In a second study, the combined effects of a mixture of xenobiotics (Atrazine® and bisphénol A) were tested on two different cell lines, hepatocytes and embryonic renal cells (E293).
The activity of cytochromes P450 (CYP) (1A1 and 3A4, enzymes involved in the detoxification process) was measured, as well as that of the glutathione-S-transferase  in order to investigate the mechanisms of the action of the formulae. The trial demonstrated that the herbal mixture of Greater Celandine (Chelidonium majus), Radish (Raphanus sativus), and Buckthorn (Frangula alnus) restores the CYP1A2 activity damaged by Roundup®, but has only a minor effect on the CYP3A4, and no effect on glutathion S-transferase.
These primary results show that environmental pollutants have intracellular effects which could be prevented by specific mixtures of plants. These effects might be attributed to the modulation of cytochromes P450, which are “detoxifying” proteins in charge of toxins. This study was the subject of a publication in an international journal : Defined plant extracts can Project human cells against combined xenobiotic effets, Gasnier et al. Journal of Occupationnal Medecine and Toxicology 2011 ; 6:3.
Also, plant mixtures tested in scientific trials have shown no cell toxicity.
 Glutathione-S-transferase represent a family of enzymes which plays an important role in the detoxification of toxic compounds of both exogenous and endogenous origins.